Plasmid Preparation:Article Title: TRB3 augments IL1β-TLR4 signaling by engaging Flightless-homolog 1
Article Snippet: .. Plasmids encoding HA-tagged LRR (leucine-rich-repeat) domains of LRRFIP2 (Addgene plasmid # 21152), and Fli1 (Addgene plasmid # 21151), were a gift from Dr. Junying Yuan (Harvard University, Boston MA), and Flag-MyD88 (Addgene plasmid # 13093) was a gift from Dr. Ruslan Medzhitov (HHMI, Yale School of Medicine). pcDNA3-HA-Fli1 was generated by PCR-based amplification of full-length, mouse Fli1, using pCMV-Sport6 mFli1 (Horizon Discovery, Denver CO) as template, and cloned into pcDNA3.1 vector using HindIII and Xho1 sites, in frame with a C-terminal HA tag. .. Myc-APEX2-TRB3 was generated by PCR amplification of APEX2 using pcDNA3-APEX2-NES as template (Addgene plasmid #49386, kind gift of Alice Ting, Stanford University, Palo Alto, CA), and subcloned into BamH1 and Xho1 sites of pcDNA3 Myc plasmid, followed by subcloning of TRB3 in frame with APEX2, using EcoRI and XbaI sites).
Article Title: Stress-induced pseudokinase TRB3 augments IL1β signaling by interacting with Flightless homolog 1.
Article Snippet: .. Plasmids encoding HA-tagged LRR (leucine-rich-repeat) domains of LRRFIP2 (Addgene plasmid # 21152) and Fli1 (Addgene plasmid # 21151) were a gift from Dr Junying Yuan (Harvard University), and Flag-MyD88 (Addgene plasmid # 13093) was a gift from Dr Ruslan Medzhitov (HHMI, Yale School of Medicine). pcDNA3-HAFli1 was generated by PCR-based amplification of full-length, mouse Fli1, using pCMV-Sport6 mFli1 (Horizon Discovery) as template, and cloned into pcDNA3.1 vector using HindIII and Xho1 sites, in frame with a C-terminal HA tag. .. MycAPEX2-TRB3 was generated by PCR amplification of APEX2 using pcDNA3-APEX2-NES as template (Addgene plasmid #49386, kind gift of Alice Ting, Stanford University) and subcloned into BamH1 and Xho1 sites of pcDNA3 Myc plasmid, followed by subcloning of TRB3 in frame with APEX2, using EcoRI and XbaI sites.
Article Title: Stress-induced pseudokinase TRB3 augments IL1β signaling by interacting with Flightless homolog 1
Article Snippet: .. Plasmids encoding HA-tagged LRR (leucine-rich-repeat) domains of LRRFIP2 (Addgene plasmid # 21152) and Fli1 (Addgene plasmid # 21151) were a gift from Dr Junying Yuan (Harvard University), and Flag-MyD88 (Addgene plasmid # 13093) was a gift from Dr Ruslan Medzhitov (HHMI, Yale School of Medicine). pcDNA3-HA-Fli1 was generated by PCR-based amplification of full-length, mouse Fli1, using pCMV-Sport6 mFli1 (Horizon Discovery) as template, and cloned into pcDNA3.1 vector using HindIII and Xho1 sites, in frame with a C-terminal HA tag. .. Myc-APEX2-TRB3 was generated by PCR amplification of APEX2 using pcDNA3-APEX2-NES as template (Addgene plasmid #49386, kind gift of Alice Ting, Stanford University) and subcloned into BamH1 and Xho1 sites of pcDNA3 Myc plasmid, followed by subcloning of TRB3 in frame with APEX2, using EcoRI and XbaI sites.
Generated:Article Title: TRB3 augments IL1β-TLR4 signaling by engaging Flightless-homolog 1
Article Snippet: .. Plasmids encoding HA-tagged LRR (leucine-rich-repeat) domains of LRRFIP2 (Addgene plasmid # 21152), and Fli1 (Addgene plasmid # 21151), were a gift from Dr. Junying Yuan (Harvard University, Boston MA), and Flag-MyD88 (Addgene plasmid # 13093) was a gift from Dr. Ruslan Medzhitov (HHMI, Yale School of Medicine). pcDNA3-HA-Fli1 was generated by PCR-based amplification of full-length, mouse Fli1, using pCMV-Sport6 mFli1 (Horizon Discovery, Denver CO) as template, and cloned into pcDNA3.1 vector using HindIII and Xho1 sites, in frame with a C-terminal HA tag. .. Myc-APEX2-TRB3 was generated by PCR amplification of APEX2 using pcDNA3-APEX2-NES as template (Addgene plasmid #49386, kind gift of Alice Ting, Stanford University, Palo Alto, CA), and subcloned into BamH1 and Xho1 sites of pcDNA3 Myc plasmid, followed by subcloning of TRB3 in frame with APEX2, using EcoRI and XbaI sites).
Article Title: Stress-induced pseudokinase TRB3 augments IL1β signaling by interacting with Flightless homolog 1.
Article Snippet: .. Plasmids encoding HA-tagged LRR (leucine-rich-repeat) domains of LRRFIP2 (Addgene plasmid # 21152) and Fli1 (Addgene plasmid # 21151) were a gift from Dr Junying Yuan (Harvard University), and Flag-MyD88 (Addgene plasmid # 13093) was a gift from Dr Ruslan Medzhitov (HHMI, Yale School of Medicine). pcDNA3-HAFli1 was generated by PCR-based amplification of full-length, mouse Fli1, using pCMV-Sport6 mFli1 (Horizon Discovery) as template, and cloned into pcDNA3.1 vector using HindIII and Xho1 sites, in frame with a C-terminal HA tag. .. MycAPEX2-TRB3 was generated by PCR amplification of APEX2 using pcDNA3-APEX2-NES as template (Addgene plasmid #49386, kind gift of Alice Ting, Stanford University) and subcloned into BamH1 and Xho1 sites of pcDNA3 Myc plasmid, followed by subcloning of TRB3 in frame with APEX2, using EcoRI and XbaI sites.
Article Title: Stress-induced pseudokinase TRB3 augments IL1β signaling by interacting with Flightless homolog 1
Article Snippet: .. Plasmids encoding HA-tagged LRR (leucine-rich-repeat) domains of LRRFIP2 (Addgene plasmid # 21152) and Fli1 (Addgene plasmid # 21151) were a gift from Dr Junying Yuan (Harvard University), and Flag-MyD88 (Addgene plasmid # 13093) was a gift from Dr Ruslan Medzhitov (HHMI, Yale School of Medicine). pcDNA3-HA-Fli1 was generated by PCR-based amplification of full-length, mouse Fli1, using pCMV-Sport6 mFli1 (Horizon Discovery) as template, and cloned into pcDNA3.1 vector using HindIII and Xho1 sites, in frame with a C-terminal HA tag. .. Myc-APEX2-TRB3 was generated by PCR amplification of APEX2 using pcDNA3-APEX2-NES as template (Addgene plasmid #49386, kind gift of Alice Ting, Stanford University) and subcloned into BamH1 and Xho1 sites of pcDNA3 Myc plasmid, followed by subcloning of TRB3 in frame with APEX2, using EcoRI and XbaI sites.
Polymerase Chain Reaction:Article Title: TRB3 augments IL1β-TLR4 signaling by engaging Flightless-homolog 1
Article Snippet: .. Plasmids encoding HA-tagged LRR (leucine-rich-repeat) domains of LRRFIP2 (Addgene plasmid # 21152), and Fli1 (Addgene plasmid # 21151), were a gift from Dr. Junying Yuan (Harvard University, Boston MA), and Flag-MyD88 (Addgene plasmid # 13093) was a gift from Dr. Ruslan Medzhitov (HHMI, Yale School of Medicine). pcDNA3-HA-Fli1 was generated by PCR-based amplification of full-length, mouse Fli1, using pCMV-Sport6 mFli1 (Horizon Discovery, Denver CO) as template, and cloned into pcDNA3.1 vector using HindIII and Xho1 sites, in frame with a C-terminal HA tag. .. Myc-APEX2-TRB3 was generated by PCR amplification of APEX2 using pcDNA3-APEX2-NES as template (Addgene plasmid #49386, kind gift of Alice Ting, Stanford University, Palo Alto, CA), and subcloned into BamH1 and Xho1 sites of pcDNA3 Myc plasmid, followed by subcloning of TRB3 in frame with APEX2, using EcoRI and XbaI sites).
Article Title: Stress-induced pseudokinase TRB3 augments IL1β signaling by interacting with Flightless homolog 1.
Article Snippet: .. Plasmids encoding HA-tagged LRR (leucine-rich-repeat) domains of LRRFIP2 (Addgene plasmid # 21152) and Fli1 (Addgene plasmid # 21151) were a gift from Dr Junying Yuan (Harvard University), and Flag-MyD88 (Addgene plasmid # 13093) was a gift from Dr Ruslan Medzhitov (HHMI, Yale School of Medicine). pcDNA3-HAFli1 was generated by PCR-based amplification of full-length, mouse Fli1, using pCMV-Sport6 mFli1 (Horizon Discovery) as template, and cloned into pcDNA3.1 vector using HindIII and Xho1 sites, in frame with a C-terminal HA tag. .. MycAPEX2-TRB3 was generated by PCR amplification of APEX2 using pcDNA3-APEX2-NES as template (Addgene plasmid #49386, kind gift of Alice Ting, Stanford University) and subcloned into BamH1 and Xho1 sites of pcDNA3 Myc plasmid, followed by subcloning of TRB3 in frame with APEX2, using EcoRI and XbaI sites.
Article Title: Stress-induced pseudokinase TRB3 augments IL1β signaling by interacting with Flightless homolog 1
Article Snippet: .. Plasmids encoding HA-tagged LRR (leucine-rich-repeat) domains of LRRFIP2 (Addgene plasmid # 21152) and Fli1 (Addgene plasmid # 21151) were a gift from Dr Junying Yuan (Harvard University), and Flag-MyD88 (Addgene plasmid # 13093) was a gift from Dr Ruslan Medzhitov (HHMI, Yale School of Medicine). pcDNA3-HA-Fli1 was generated by PCR-based amplification of full-length, mouse Fli1, using pCMV-Sport6 mFli1 (Horizon Discovery) as template, and cloned into pcDNA3.1 vector using HindIII and Xho1 sites, in frame with a C-terminal HA tag. .. Myc-APEX2-TRB3 was generated by PCR amplification of APEX2 using pcDNA3-APEX2-NES as template (Addgene plasmid #49386, kind gift of Alice Ting, Stanford University) and subcloned into BamH1 and Xho1 sites of pcDNA3 Myc plasmid, followed by subcloning of TRB3 in frame with APEX2, using EcoRI and XbaI sites.
Amplification:Article Title: TRB3 augments IL1β-TLR4 signaling by engaging Flightless-homolog 1
Article Snippet: .. Plasmids encoding HA-tagged LRR (leucine-rich-repeat) domains of LRRFIP2 (Addgene plasmid # 21152), and Fli1 (Addgene plasmid # 21151), were a gift from Dr. Junying Yuan (Harvard University, Boston MA), and Flag-MyD88 (Addgene plasmid # 13093) was a gift from Dr. Ruslan Medzhitov (HHMI, Yale School of Medicine). pcDNA3-HA-Fli1 was generated by PCR-based amplification of full-length, mouse Fli1, using pCMV-Sport6 mFli1 (Horizon Discovery, Denver CO) as template, and cloned into pcDNA3.1 vector using HindIII and Xho1 sites, in frame with a C-terminal HA tag. .. Myc-APEX2-TRB3 was generated by PCR amplification of APEX2 using pcDNA3-APEX2-NES as template (Addgene plasmid #49386, kind gift of Alice Ting, Stanford University, Palo Alto, CA), and subcloned into BamH1 and Xho1 sites of pcDNA3 Myc plasmid, followed by subcloning of TRB3 in frame with APEX2, using EcoRI and XbaI sites).
Article Title: Stress-induced pseudokinase TRB3 augments IL1β signaling by interacting with Flightless homolog 1.
Article Snippet: .. Plasmids encoding HA-tagged LRR (leucine-rich-repeat) domains of LRRFIP2 (Addgene plasmid # 21152) and Fli1 (Addgene plasmid # 21151) were a gift from Dr Junying Yuan (Harvard University), and Flag-MyD88 (Addgene plasmid # 13093) was a gift from Dr Ruslan Medzhitov (HHMI, Yale School of Medicine). pcDNA3-HAFli1 was generated by PCR-based amplification of full-length, mouse Fli1, using pCMV-Sport6 mFli1 (Horizon Discovery) as template, and cloned into pcDNA3.1 vector using HindIII and Xho1 sites, in frame with a C-terminal HA tag. .. MycAPEX2-TRB3 was generated by PCR amplification of APEX2 using pcDNA3-APEX2-NES as template (Addgene plasmid #49386, kind gift of Alice Ting, Stanford University) and subcloned into BamH1 and Xho1 sites of pcDNA3 Myc plasmid, followed by subcloning of TRB3 in frame with APEX2, using EcoRI and XbaI sites.
Article Title: Stress-induced pseudokinase TRB3 augments IL1β signaling by interacting with Flightless homolog 1
Article Snippet: .. Plasmids encoding HA-tagged LRR (leucine-rich-repeat) domains of LRRFIP2 (Addgene plasmid # 21152) and Fli1 (Addgene plasmid # 21151) were a gift from Dr Junying Yuan (Harvard University), and Flag-MyD88 (Addgene plasmid # 13093) was a gift from Dr Ruslan Medzhitov (HHMI, Yale School of Medicine). pcDNA3-HA-Fli1 was generated by PCR-based amplification of full-length, mouse Fli1, using pCMV-Sport6 mFli1 (Horizon Discovery) as template, and cloned into pcDNA3.1 vector using HindIII and Xho1 sites, in frame with a C-terminal HA tag. .. Myc-APEX2-TRB3 was generated by PCR amplification of APEX2 using pcDNA3-APEX2-NES as template (Addgene plasmid #49386, kind gift of Alice Ting, Stanford University) and subcloned into BamH1 and Xho1 sites of pcDNA3 Myc plasmid, followed by subcloning of TRB3 in frame with APEX2, using EcoRI and XbaI sites.
Clone Assay:Article Title: TRB3 augments IL1β-TLR4 signaling by engaging Flightless-homolog 1
Article Snippet: .. Plasmids encoding HA-tagged LRR (leucine-rich-repeat) domains of LRRFIP2 (Addgene plasmid # 21152), and Fli1 (Addgene plasmid # 21151), were a gift from Dr. Junying Yuan (Harvard University, Boston MA), and Flag-MyD88 (Addgene plasmid # 13093) was a gift from Dr. Ruslan Medzhitov (HHMI, Yale School of Medicine). pcDNA3-HA-Fli1 was generated by PCR-based amplification of full-length, mouse Fli1, using pCMV-Sport6 mFli1 (Horizon Discovery, Denver CO) as template, and cloned into pcDNA3.1 vector using HindIII and Xho1 sites, in frame with a C-terminal HA tag. .. Myc-APEX2-TRB3 was generated by PCR amplification of APEX2 using pcDNA3-APEX2-NES as template (Addgene plasmid #49386, kind gift of Alice Ting, Stanford University, Palo Alto, CA), and subcloned into BamH1 and Xho1 sites of pcDNA3 Myc plasmid, followed by subcloning of TRB3 in frame with APEX2, using EcoRI and XbaI sites).
Article Title: Stress-induced pseudokinase TRB3 augments IL1β signaling by interacting with Flightless homolog 1.
Article Snippet: .. Plasmids encoding HA-tagged LRR (leucine-rich-repeat) domains of LRRFIP2 (Addgene plasmid # 21152) and Fli1 (Addgene plasmid # 21151) were a gift from Dr Junying Yuan (Harvard University), and Flag-MyD88 (Addgene plasmid # 13093) was a gift from Dr Ruslan Medzhitov (HHMI, Yale School of Medicine). pcDNA3-HAFli1 was generated by PCR-based amplification of full-length, mouse Fli1, using pCMV-Sport6 mFli1 (Horizon Discovery) as template, and cloned into pcDNA3.1 vector using HindIII and Xho1 sites, in frame with a C-terminal HA tag. .. MycAPEX2-TRB3 was generated by PCR amplification of APEX2 using pcDNA3-APEX2-NES as template (Addgene plasmid #49386, kind gift of Alice Ting, Stanford University) and subcloned into BamH1 and Xho1 sites of pcDNA3 Myc plasmid, followed by subcloning of TRB3 in frame with APEX2, using EcoRI and XbaI sites.
Article Title: Stress-induced pseudokinase TRB3 augments IL1β signaling by interacting with Flightless homolog 1
Article Snippet: .. Plasmids encoding HA-tagged LRR (leucine-rich-repeat) domains of LRRFIP2 (Addgene plasmid # 21152) and Fli1 (Addgene plasmid # 21151) were a gift from Dr Junying Yuan (Harvard University), and Flag-MyD88 (Addgene plasmid # 13093) was a gift from Dr Ruslan Medzhitov (HHMI, Yale School of Medicine). pcDNA3-HA-Fli1 was generated by PCR-based amplification of full-length, mouse Fli1, using pCMV-Sport6 mFli1 (Horizon Discovery) as template, and cloned into pcDNA3.1 vector using HindIII and Xho1 sites, in frame with a C-terminal HA tag. .. Myc-APEX2-TRB3 was generated by PCR amplification of APEX2 using pcDNA3-APEX2-NES as template (Addgene plasmid #49386, kind gift of Alice Ting, Stanford University) and subcloned into BamH1 and Xho1 sites of pcDNA3 Myc plasmid, followed by subcloning of TRB3 in frame with APEX2, using EcoRI and XbaI sites.
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